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KMID : 0861020220370050017
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Korea Journal of Herbology
2022 Volume.37 No. 5 p.17 ~ p.26
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Combined Treatment of Silymarin and Jakyakgamcho-tang Suppresses Hepatic Lipid Accumulation and Inflammation in C57BL/6 Mice
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Choi Jeong-Won
Cho Su-Jung
Shin Mi-Rae
Park Hae-Jin
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Abstract
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Objective : The aim of the present study is to examine hepatic lipid-lowering and anti-inflammatory effects of silymarin
combined with Jakyakgamcho-tang on non-alcoholic fatty liver disease in a high fat diet-induced obese mice model.
Methods : C57BL/6 mice were divided into four dietary groups: (1) Normal, (2) Control (60% high-fat diet), (3)
Control + silymarin 50 mg/kg/day (Silymarin), (4) Control + Silymarin 50 mg/kg/day + Jakyakgamcho-tang 100
mg/kg/day (SPG). After 12 weeks administration, mice were sacrificed and lipids and inflammation-related biomarkers were analyzed liver and plasma.
Results : Silymarin and SPG treatments significantly lowered body and liver weights compared to the Control. Serumlipids (triglyceride (TG), total cholesterol) and pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin
1β, and IL-6) concentrations were significantly lowered in the Silymarin and SPG groups than the Control group.
Silymarin and SPG treatments suppressed hepatic TG level and hepatic lipid droplets compared to the Control. Theses
two treatments significantly increased hepatic kinase B1 and AMP-activated protein kinase protein levels, and
significantly decreased hepatic key lipogenic enzymes (acetyl-CoA carboxylase, fatty acid synthase and stearyl coenzyme A desaturase 1) protein levels than the Control. SPG also significantly increased hepatic fatty acid oxidation-related
protein (peroxisome proliferator-activated receptor alpha and uncoupling protein 2) levels than the Control.
Conclusions: Silymarin and SPG suppressed hepatic lipid accumulation by regulating hepatic protein expression, and
lowered blood pro-inflammatory cytokines concentrations though the synergic effect of silymarin and Jakyakgamchotang was not clear.
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KEYWORD
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silymarin, Jakyakgamcho-tang, hepatic lipid accumulation, inflammation, C57BL/6 mice
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